mwambo oligo process workstation

mwambo oligo process workstation,
oligo process aupport zida,

Gawo la Elution

1. Mzere wa mabowo 8 a jakisoni wamadzimadzi, omwe amatha kubayidwa nthawi imodzi, ndipo madzi ofunikira amatha kuwongoleredwa payekhapayekha.
2. 5 malo a botolo la reagent, botolo lirilonse liri ndi botolo la 4L reagent, ndiko kuti, likhoza kukhala logwirizana ndi mitundu 5 ya reagents.
3. Chidacho chimayendetsedwa ndi madzi otsekemera, omwe ndi abwino komanso osinthika kuti azitha kuyendetsa madzi othamanga.
4. Zidazi zili ndi malo a mbale ya 96, ndipo mtundu wa mbale umatchulidwa ndi kasitomala.
5. Chimbale chokhala ndi chitsime chakuya cha 96 chikhoza kuikidwa pansi pa mbale kuti chitenge madzi, kapena chikhoza kulumikizidwa mwachindunji ndi payipi kuti chichotse madzi otayika.
6. Mu gawo la mapulogalamu, mukhoza kusankha mtundu wa reagent ndi kuchuluka kwa reagent kuti muwonjezere zitsanzo, nthawi ikhoza kukhazikitsidwa, makina oponderezedwa akhoza kukhazikitsidwa, nthawi yokakamiza ikhoza kuyendetsedwa, ndipo masitepe akhoza kukhazikitsidwa. kuthamanga mosalekeza.2 mitundu ya maphikidwe.

Chiyeretso gawo

1. Zida zimatha kudzaza mpaka 96 c18 mizati nthawi imodzi.
2. Zida zokhazikika zimakhala ndi madoko asanu a reagent, chogwiritsira ntchito chubu cha centrifuge, ndipo mwiniwakeyo amatha kunyamula machubu a 96 primer centrifuge.
3. Zidazi zimagwiritsa ntchito njira yodziyimira pawokha yodziyimira patatu, yolondola kwambiri komanso yobwereza bwino.
4. The zida utenga kuyamwa fyuluta mtundu.
5. The zoikamo mapulogalamu ndi kusintha ndi yosavuta ntchito.
6. Mutu woyamwa uli ndi chipangizo chamadzimadzi chomwe chimatha kuzindikira kutalika kwa msinkhu wamadzimadzi.

Chigawo cha bomba

1. Chitsime cha 96 chopita ku chubu cha centrifuge.
2. Zodziwikiratu za pipette: choyambira chilichonse chikhoza kugawidwa m'mizere iwiri kwambiri.
3. X, Y, Z kuyenda mozungulira.
4. Kuchuluka kwa voliyumu yoyamwa (5-200ul).
5. Pogawanika kukhala machubu angapo.
6. Zofunikira pa nthawi.
7. Ikhoza kulumikizidwa ndi template yoyezera, Kulakwitsa kofulumira, pali kuyimitsa pakati pa Red kumasonyeza kuti voliyumu yonse sikwanira ndipo ikufunika kuti ikhale yowonjezera.
Pali nsonga zofiira, zachikasu ndi zobiriwira zowerengera voliyumu: chikasu chimatanthawuza kuti voliyumu ndi yocheperako, mutha kusintha voliyumu pamanja, osafunikira kuphatikizika, zobiriwira zimatanthawuza zachilendo.
8. Zambiri monga nambala yazambiri, kuchuluka kwa machubu, ndi kuchuluka kwa chubu chilichonse zimawonetsedwa podzaza chitsanzo chilichonse.Maonekedwe akuwonetsa kuti zoyambira zagawika ndipo sizinagawidwe, zowongolera zokha zisanaperekedwe.

chizolowezi cha oligo process workstation cha oligo process aupport zida, chili ndi ntchito ya Pepetting, kusakaniza, kugwedeza, kutentha ndi zina.