Zida Zoyeretsera Zoyeretsera Oligo
Kufotokozera
| Ma board ogwirizana | 1, 3, 5, 8. |
| Sefa | kusefedwa kuwomba, kusefera koyamwa |
| Chiwerengero cha madoko a jakisoni | 5, 6, 7, 8, 9, 10. |
| Mitundu ya mbale yogwirizana | C18 mbale, mbale yakuya bwino, mbale yopanga (yogwirizana ndi mbale zambiri zopangidwa), mbale ya microtiter |
| Module | nkhwangwa imodzi kapena mbali ziwiri |
| Voteji | 220V |
| Chitsimikizo | 1 chaka |
| Mwambo | Adalandiridwa |
Njira Zosiyanasiyana Zoyeretsera
1. Gel Electrophoresis Chromatography Kuyeretsedwa
Gwiritsani ntchito denaturing polyacrylamide gel electrophoresis chromatography kuti muyeretsedwe.Denaturing wothandizira nthawi zambiri amakhala 4M formamide kapena 7M urea, kuchuluka kwa acrylamide kumakhala pakati pa 5-15%, ndipo gawo la methacrylamide limakhala pakati pa 2-10%.
Pambuyo pa electrophoresis, malo a gulu la nucleic acid ayenera kutsimikiziridwa pansi pa kuyatsa kwa kuwala kwa ultraviolet, gel osakaniza omwe ali ndi chandamale cha nucleic acid amadulidwa, nucleic acid imaphwanyidwa ndikuphwanyidwa, ndiyeno njira yothetsera leaching imayikidwa, imachotsedwa. quantified ndi lyophilized.
2. DMT-On, HPLC Kuyeretsa
Sankhani DMT-On akafuna pa kaphatikizidwe, zopangira anali centrifuged ndi anaikira firiji kuchotsa owonjezera ammonia pambuyo aminolysis.
Kulekanitsa kunkachitika pogwiritsa ntchito khola la C18 ndi acetonitrile ndi 10% triethylamine-acetic acid (TEAA) monga momwe zimawonekera.Pambuyo pakutha, imayikidwa, ndiyeno gulu la DMT limachotsedwa ndi trifluoroacetic acid.Pambuyo pa neutralization, mchere wina ndi mamolekyu ang'onoang'ono amachotsedwa kudzera mu chubu chodulidwa, ndipo pamapeto pake amachotsedwa.
Njirayi ingapeze mankhwala ndi chiyero chapamwamba, koma m'pofunika kumvetsera zochitika za depurination.
3. DMT-Off, HPLC Kuyeretsa
Sankhani DMT-Off pa kaphatikizidwe, ndi mankhwala osakhwima anali centrifuged ndi anaikira firiji kuchotsa owonjezera ammonia pambuyo ammonolysis.
Kulekanitsa kunachitika pogwiritsa ntchito chigawo cha C18 chokhala ndi acetonitrile ndi 10% triethylamine-acetic acid m'madzi monga momwe zimawonekera.Kupatukana kutatha ndikuwerengedwa, ma aliquots amakhala lyophilized.
Njirayi imafuna kusintha kosamalitsa kwa mikhalidwe yolekanitsa, ndipo imathanso kupeza mamolekyu abwino kwambiri.
