Misampha ya mamolekyulu a phosphoramidite ndi ma reagents

Ntchito:

Msampha wa Molecular umagwiritsidwa ntchito kutsatsa madzi mu reagents ndi amidite, poyamba adapangidwa kuti apange oligonucleotides.Ndi yabwino, yopanda fumbi, komanso yopanda flannel.Ikhoza kuwonjezeredwa ku mitundu yosiyanasiyana ya solvents ndi organic solutions kuchotsa kuchuluka kwa madzi.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Mafotokozedwe Akatundu

Mu mayeso a kaphatikizidwe a ABI ndi MilliGen/PerSeptive DNA, paketi ya sub-sieve imatha kusunga madzi mu mabotolo a nitrile ndi activator pansi pa 10 ppm, popanda kudandaula za valavu kapena valavu ya throttle kugawidwa mu sub-sieve.Fumbi kapena lint pa thumba lazoyikapo latsekedwa.Itha kugwiritsidwa ntchito mwachindunji kutsitsa madzi a 500 ml, 1L, 2 L ndi mabotolo ena osungunulira, komanso mafotokozedwe a paketi ya sub-sieve amathanso makonda.

Zotsatirazi zikuwonetsa mphamvu yowonongeka ya 10 g msampha wa maselo mu 500ml, 1L, 4L nitrile zitsanzo zokhala ndi madzi osiyanasiyana.

Fananizani ndi import molecular trap

500 ml 197 ppm ACN
Nthawi(h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Mayiko Ena	197	43	27	15	15

1 L 143 ppm ACN
Nthawi(h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Mayiko Ena	142	47	36	23	15

4 L 141 ppm ACN
Nthawi(h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Mayiko Ena	141	96	95	85	72

Malangizo ndi kugwiritsa ntchito

Msampha wa mamolekyu ndi wodzaza ndi vacuum, ndipo uyenera kutsegulidwa ngakhale utagwiritsidwa ntchito, ndipo chisindikizo cha botolo la reagent chiyenera kutsimikiziridwa pakagwiritsidwa ntchito.
2g sieve kwa maola 24 imatha kuchepetsa 165 ppm madzi mu 500 ml nitrile kufika 105 ppm.
5 g sieve kwa maola 24 imatha kuchepetsa 172 ppm madzi mu 500 ml nitrile kufika 58 ppm.
10 g sieve 24 h Ikhoza kuchepetsa madzi okwanira 166 ppm mu 1 L nitrile kufika 68 ppm.
20 g subseve imatha kuchepetsa 162 ppm madzi mu 4 L nitrile kufika 109 ppm kwa 24 h.

Timalimbikitsa 2 g subsieve mabotolo 50-250ml reagent, 5g mabotolo 250-500ml reagent, 10g mabotolo 500-1000ml reagent, ndi 20g 1000-2000ml mabotolo reagent.

Chitsimikizo

Phukusi lililonse laling'ono lachiwonetsero limayang'aniridwa bwino kwambiri panthawi yopanga.Ndipo ndikofunikira kuti makasitomala ayang'ane zonyamula musanagwiritse ntchito:

Choyamba, tsimikizirani kuti vacuum yopakirayo ilibe.Kutayikira kulikonse kwa vacuum kapena kulowa kwa mpweya kumachepetsa magwiridwe antchito.

Chachiwiri, samalani potsegula zoyikapo kuti filimu yoyika pakompyuta yapang'onopang'ono isasokonezedwe, ndipo onani ngati filimuyo ili Pali kutayikira.

Thandizo pambuyo pa ntchito

Sizowopsa kapena zovulaza, zidzaipitsidwa ndi mankhwala ndi zosungunulira zomwe amakumana nazo ndipo ziyenera kutayidwa ngati zinyalala zoipitsidwa zikagwiritsidwa ntchito.

Chitsanzo ndi ntchito

Tapereka kale matumba a sub-sieve kukula kwake kwa 2 g, 5 g, 10 g, ndi 20 g., ndi zina zitha kusinthidwa malinga ndi zosowa za makasitomala.

The molecular msampha ndi oyenera kwa nthawi yaitali kukhudzana nitrile, ndipo angagwiritsidwenso ntchito asidi asidi, etha, acetic efa, butyl acetate, mowa, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, asidi nitric, phosphoric acid. , sulfuric acid, methyl chloride, nitrogen methyl imidazole, etc.

Zingakhale zosayenera kwa nthawi yayitali kukhudzana ndi tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) ndi njira zina.